Consider a laboratory experiment that investigates the ability of an enzyme to hydrolyse protein at different pH levels. The solutions in the different test tubes are all at 37 °C and may contain distilled water, protein and enzyme and be at different pH. Which test tubes are likely to be the controls?

The ones containing distilled water
The test tubes with pepsin added
The test tube with a pH of 7.0
The ones without any added enzyme
The ones without any added enzyme  Such an investigation should have one tube that contains the enzyme (e.g. pepsin) and another lacking the enzyme, with the tubes being identical in all other respects (i.e. both containing protein dissolved in distilled water at the same pH). In this case the one(s) without the enzyme is the control.

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